1WL8
Crystal structure of PH1346 protein from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-21 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.808, 63.480, 65.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.990 - 1.450 |
| R-factor | 0.15638 |
| Rwork | 0.155 |
| R-free | 0.17365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qdl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.250 |
| Number of reflections | 33220 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.8 | 295 | Acetate, PEG, pH 4.8, microbatch, temperature 295.0K |
