1WKV
Crystal structure of O-phosphoserine sulfhydrylase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.874, 74.874, 275.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.278 |
| Number of reflections | 52917 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 97.3 | 82.4 |
| Redundancy | 6.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | pyridoxal 5'-phosphate, 2-mercaptoethanol, cacodylate, sodium acetate, PEG 8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
