1WKU
High resolution structure of the human alpha-actinin isoform 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.499, 45.960, 66.998 |
| Unit cell angles | 86.67, 87.80, 87.33 |
Refinement procedure
| Resolution | 33.420 - 1.600 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LOW RESOLUTION MODEL OF DIFFERENT SPACE GROUP |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.420 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 56760 | |
| Completeness [%] | 94.0 | 87.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | peg 20000, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
