1WKQ
Crystal Structure of Bacillus subtilis Guanine Deaminase. The first domain-swapped structure in the cytidine deaminase superfamily
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0, 0.9798, 0.9799, 0.9571 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.566, 91.267, 80.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.170 |
| Rwork | 0.161 |
| R-free | 0.17700 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.057 | 0.280 |
| Number of reflections | 99896 | |
| <I/σ(I)> | 36.3 | 3.8 |
| Completeness [%] | 93.9 | 59 |
| Redundancy | 10.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | polyethylene glycol 4000, ammonium acetate, sodium citrate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
