1WFC
STRUCTURE OF APO, UNPHOSPHORYLATED, P38 MITOGEN ACTIVATED PROTEIN KINASE P38 (P38 MAP KINASE) THE MAMMALIAN HOMOLOGUE OF THE YEAST HOG1 PROTEIN
Experimental procedure
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-14 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.193, 86.250, 123.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.29700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.000 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.300 * | 2.300 * |
| Rmerge | 0.057 | |
| Total number of observations | 43442 * | |
| Number of reflections | 19927 | |
| Completeness [%] | 89.6 | 85 * |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 10 | 1 | reservoir | sodium citrate | 0.56 (M) | |
| 11 | 1 | reservoir | ammonium sulfate | 130 (mM) | |
| 2 | 1 | drop | HEPES | 30 (mM) | |
| 3 | 1 | drop | DTT | 6 (mM) | |
| 4 | 1 | drop | 0.06 (M) | ||
| 5 | 1 | drop | glycerol | 3 (%) | |
| 6 | 1 | drop | Na HEPES | 16 (mM) | |
| 7 | 1 | drop | sodium citrate | 0.224 (M) | |
| 8 | 1 | drop | ammonium sulfate | 52 (mM) | |
| 9 | 1 | reservoir | Na HEPES | 40 (mM) |
