1WFC

STRUCTURE OF APO, UNPHOSPHORYLATED, P38 MITOGEN ACTIVATED PROTEIN KINASE P38 (P38 MAP KINASE) THE MAMMALIAN HOMOLOGUE OF THE YEAST HOG1 PROTEIN

Experimental procedure

Temperature [K]	77
Detector technology	IMAGE PLATE
Collection date	1996-05-14
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	45.193, 86.250, 123.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.213
Rwork	0.213
R-free	0.29700
RMSD bond length	0.010
RMSD bond angle	24.000 *
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.057
Total number of observations	43442 *
Number of reflections	19927
Completeness [%]	89.6	85 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
10	1	reservoir	sodium citrate	0.56 (M)
11	1	reservoir	ammonium sulfate	130 (mM)
2	1	drop	HEPES	30 (mM)
3	1	drop	DTT	6 (mM)
4	1	drop		0.06 (M)
5	1	drop	glycerol	3 (%)
6	1	drop	Na HEPES	16 (mM)
7	1	drop	sodium citrate	0.224 (M)
8	1	drop	ammonium sulfate	52 (mM)
9	1	reservoir	Na HEPES	40 (mM)