1WE1
Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC6803 in complex with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.790, 113.730, 109.700 |
| Unit cell angles | 90.00, 112.26, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.224 |
| Rwork | 0.220 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dve |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.214 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 46584 | |
| <I/σ(I)> | 7.2 | 2.4 |
| Completeness [%] | 94.7 | 83.2 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 400, 2-propanol, 1,5-diaminopentane dihydrochloride, sodium citrate, pottasium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
