1WDW

Structural basis of mutual activation of the tryptophan synthase a2b2 complex from a hyperthermophile, Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Detector	OXFORD PX210
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	89.056, 220.257, 292.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.170 - 3.000
R-factor	0.196
Rwork	0.196
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v8z 1geq
RMSD bond length	0.008
RMSD bond angle	1.300
Data scaling software	d*TREK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.170
High resolution limit [Å]	3.000	3.000
Rmerge	0.091	0.367
Number of reflections	115486
Completeness [%]	99.6	99.8
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	sodium citrate, PEG 8000, potassium acetate, ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K