1WD6
crystal structure of JW1657 from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9640, 0.97914, 0.97964 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.803, 85.803, 95.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.370 - 2.900 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 9408 | |
| Completeness [%] | 99.7 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil Batch | 7 | 293 | Ammonium Sulfate, PEG8000, pH 7.0, Oil Batch, temperature 293K |
