1WD1
Crystal structures of the hyperthermophilic chromosomal protein Sac7d in complex with DNA decamers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.634, 46.841, 53.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.25 |
| Rwork | 0.248 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb 1azp |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | 0.540 |
| Number of reflections | 5166 | |
| <I/σ(I)> | 25 | 2 |
| Completeness [%] | 98.0 | 88.5 |
| Redundancy | 4.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | potassium phosphate, PEG8000, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium phosphate | ||
| 2 | 1 | 1 | PEG8000 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | potassium phosphate | ||
| 5 | 1 | 2 | PEG8000 | ||
| 6 | 1 | 2 | H2O |
