1WBA
WINGED BEAN ALBUMIN 1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 286 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-12 |
| Detector | WEISSENBERG |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 95.600, 95.600, 86.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT AND MOLECULAR REPLACEMENT |
| Starting model (for MR) | SOYBEAN TRYPSIN INHIBITOR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.874 * |
| Data reduction software | Agrovata |
| Data scaling software | Agrovata |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.092 |
| Number of reflections | 12039 |
| <I/σ(I)> | 4.2 |
| Completeness [%] | 80.0 |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.5 | VAPOR DIFFUSION FROM PROTEIN AT 5MG/ML IN 0.1M SUCCINATE BUFFER PH 5.5 EQUILIBRATED AGAINST 30% ETHANOL., vapor diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ethanol | 30 (%) |
