1WAU
Structure of KDPG Aldolase E45N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 105.195, 105.195, 51.444 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 91.290 - 2.800 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eua |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.311 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.550 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 15069 | |
| <I/σ(I)> | 8.5 | 1.6 |
| Completeness [%] | 96.1 | 93.8 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M AMMONIUM SULPHATE, 15% PEG4000, 10MM TRIS, PH8.0, pH 8.00 |
