1WAL

3-ISOPROPYLMALATE DEHYDROGENASE (IPMDH) MUTANT (M219A)FROM THERMUS THERMOPHILUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 32 2 1
Unit cell lengths	78.400, 78.400, 158.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.270
R-factor	0.184 *
Rwork	0.184
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ipd
RMSD bond length	0.010
RMSD bond angle	1.300
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.270
Rmerge	0.055 *
Total number of observations	54255 *
Number of reflections	22587 *
Completeness [%]	84.0
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6		6 MG/ML PROTEIN IN 1.4 M AMMONIUM SULFATE, pH 7.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	ammonium sulfate	1.4 (M)
3	1	drop		20 (mM)