1WAB
PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Collection date | 1995-11-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.260, 81.260, 72.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.500 - 1.700 |
| Rwork | 0.195 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 1.650 * |
| Rmerge | 0.063 |
| Total number of observations | 387544 * |
| Number of reflections | 33716 |
| Completeness [%] | 99.7 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.15-3.15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | |
| 3 | 1 | drop | DTT | 1 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 8 (%sat) | |
| 5 | 1 | drop | sodium acetate | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 16 (%sat) | |
| 7 | 1 | reservoir | sodium acetate | 200 (mM) |
