1WA5
Structure of the Cse1:Imp-alpha:RanGTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0055 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 272.672, 72.261, 97.640 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 34.923 - 2.000 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EE4 + 1IBR |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.923 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.089 | 1.800 |
| Number of reflections | 120581 | 16799 |
| <I/σ(I)> | 5.5 | 1.9 |
| Completeness [%] | 94.3 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 50mM TrisHCl, 200mM NaCl, 5mM Mg(OAc)2, .1mM GTP, 22% PEG3350 and 2% PEG400. |
