1W97
cyto-EpsL: the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-29 |
| Wavelength(s) | 0.97951,0.97965,0.97951 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.006, 88.707, 55.371 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 17.690 - 2.700 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.054 | 0.369 |
| Number of reflections | 7476 | |
| <I/σ(I)> | 21.7 | 3.5 |
| Completeness [%] | 95.8 | 75.8 |
| Redundancy | 7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 11-15% PEG 4000, 150MM CAOAC2, 100MM TRIS PH8, 20 DEGREES C, pH 8.00 |
