1W93

Crystal Structure of Biotin Carboxylase Domain of Acetyl-Coenzyme A Carboxylase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Spacegroup name	P 62
Unit cell lengths	101.740, 101.740, 145.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.630 - 2.500
R-factor	0.254
Rwork	0.254
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL
Data scaling software	HKL
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.250
Number of reflections	24971
<I/σ(I)>	16.4	3.6
Completeness [%]	84.3	57.4
Redundancy	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1