1W8S
The mechanism of the Schiff Base Forming Fructose-1,6-bisphosphate Aldolase: Structural analysis of reaction intermediates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Collection date | 2003-11-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.500, 157.300, 101.200 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| R-factor | 0.149 |
| Rwork | 0.149 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojx |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.057 | 0.328 |
| Number of reflections | 199708 | |
| <I/σ(I)> | 22.2 | 2.2 |
| Completeness [%] | 100.0 | 60 |
| Redundancy | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 9% (W/V) PEG 4000, 0.1 M NAACETATE PH 5.0, 1-8% GLYCEROL, 100 MM FBP |
