1W8A

Third LRR domain of Drosophila Slit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	121.213, 31.852, 49.526
Unit cell angles	90.00, 100.72, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.1994
Rwork	0.199
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ozn
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.190
Number of reflections	4705
<I/σ(I)>	16.2	7
Completeness [%]	98.5	96.3
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1