1W89
Structure of the reduced form of human thioredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.580, 49.610, 80.020 |
| Unit cell angles | 87.77, 82.30, 79.31 |
Refinement procedure
| Resolution | 19.230 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uvz |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.324 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 50114 | |
| <I/σ(I)> | 11.42 | 4.29 |
| Completeness [%] | 97.1 | 97.8 |
| Redundancy | 2.68 | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | PEG 3350 22% (W/V), NA ACETATE 0.1M (PH 4.6), AMMONIUM SULFATE 0.2M, DTT 1MM. SOACKING IN A TRISHYDROXIMETHYLPHOSPHINE SOLUTION 10MM |
