1W7P
The crystal structure of endosomal complex ESCRT-II (VPS22/VPS25/VPS36)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 149.908, 149.908, 186.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.060 - 3.600 |
| R-factor | 0.294 |
| Rwork | 0.292 |
| R-free | 0.33000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.603 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.060 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 24861 | |
| <I/σ(I)> | 4.7 | 1.4 |
| Completeness [%] | 99.3 | 95.3 |
| Redundancy | 7.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 3.1% PEG35000, 0.1 M TRIS ACETATE PH 8.5, 1.36 M SODIUM FORMIATE, 19% GLYCEROL |
