1W7N

Crystal structure of human kynurenine aminotransferase I in PMP form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	AREA DETECTOR
Detector	MSC
Spacegroup name	P 32 2 1
Unit cell lengths	145.461, 145.461, 67.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.920 - 2.900
R-factor	0.172
Rwork	0.171
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w7l
RMSD bond length	0.017
RMSD bond angle	1.660
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Rmerge	0.096 *
Total number of observations	110645 *
Number of reflections	18243
Completeness [%]	99.3
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *	pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium formate	4.5 (M)
3	1	reservoir	Tris	0.1 (M)	pH7.5