1W77
2C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD) from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2003-09-26 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 74.496, 74.496, 223.026 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.950 - 2.000 |
| R-factor | 0.237 |
| Rwork | 0.232 |
| R-free | 0.34900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i52 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.652 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.430 |
| Number of reflections | 16508 | |
| <I/σ(I)> | 22.3 | 2.8 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 16.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.5, 0.05 M CADMIUM SULFATE, 1 M SODIUM ACETATE, 0.04 COPPER(II) CHLORIDE |
