1W6K

Structure of human OSC in complex with Lanosterol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	189.570, 201.472, 62.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.147
R-free	0.18800
Structure solution method	OTHER
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.490
Number of reflections	79898
<I/σ(I)>	14.4	3.1
Completeness [%]	99.2	96.3
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% (W/V) PEG3350 0.4M AMMONIUM ACETATE 0.1M TRIS PH 8.5 10% (V/V) ETHYLENE GLYCOL