1W5E
FtsZ W319Y mutant, P1 (M. jannaschii)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 113.951, 114.332, 115.135 |
| Unit cell angles | 90.07, 90.19, 119.86 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.2638 |
| Rwork | 0.264 |
| R-free | 0.29850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fsz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.258 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 85869 | |
| <I/σ(I)> | 6.6 | 1.9 |
| Completeness [%] | 94.7 | 94.7 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
