1W4R
Structure of a type II thymidine kinase with bound dTTP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794, 1.277, 0.9196 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.496, 122.920, 115.274 |
| Unit cell angles | 90.00, 130.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.830 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18900 |
| Structure solution method | MAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.950 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 140080 | |
| <I/σ(I)> | 17.1 | 7.9 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | pH 7.20 |
