1W4P
Binding of Nonnatural 3'-Nucleotides to Ribonuclease A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.451, 32.942, 73.094 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 41.390 - 1.690 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.090 | 0.120 |
| Number of reflections | 27984 | |
| <I/σ(I)> | 9.4 | 8.3 |
| Completeness [%] | 91.4 | 94.2 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20MM SODIUM CITRATE BUFFER, PH5.5, CONTAINING PEG4000 (25% W/V), pH 5.50 |
