1W46

P4 protein from Bacteriophage PHI12 in complex with ADP and MG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-10
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	105.000, 128.400, 158.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.2093
Rwork	0.209
R-free	0.25260
Structure solution method	OTHER
RMSD bond length	0.007
RMSD bond angle	1.320
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.140	0.330
Number of reflections	29666
<I/σ(I)>	16	6.5
Completeness [%]	100.0	100
Redundancy	9.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 10MM ADP 10MM MGCL, PROTEIN CONCENTRATION 10 MG/ML, SITTING DROPS, 22C