1W44
P4 protein from Bacteriophage PHI12 in complex with ADP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.185, 130.908, 158.831 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1823 |
| Rwork | 0.182 |
| R-free | 0.22770 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 68669 | |
| <I/σ(I)> | 32 | 2.4 |
| Completeness [%] | 93.0 | 97 |
| Redundancy | 24 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 10MM ADP, PROTEIN CONCENTRATION 10 MG/ML, SITTING DROP, 22C |
