1W3W
The 2.1 Angstroem resolution structure of annexin A8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-14 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.761, 65.581, 59.229 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 23.310 - 1.990 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 22712 | |
| <I/σ(I)> | 21.7 | 5.52 |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50MM CACODYLATE PH=6, 10MM CACL2, 45% (W/V) AMMONIUM SULPHATE |
