1W3Q

NimA from D. radiodurans with covalenly bound lactate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	99.803, 38.773, 60.001
Unit cell angles	90.00, 114.05, 90.00

Refinement procedure

Resolution	20.000 - 1.880
R-factor	0.172
Rwork	0.169
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w3o
RMSD bond length	0.014
RMSD bond angle	1.514
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.880
Rmerge	0.080	0.470
Number of reflections	17262
<I/σ(I)>	9.9	2.2
Completeness [%]	99.8	100
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1