1W31

YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 5-HYDROXYLAEVULINIC ACID COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-16
Detector	ADSC CCD
Spacegroup name	I 4 2 2
Unit cell lengths	102.570, 102.570, 168.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.280 - 1.900
R-factor	0.1895 *
Rwork	0.190
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ylv
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.058	0.419
Number of reflections	1067852
<I/σ(I)>	8	1.7
Completeness [%]	100.0	100
Redundancy	11.9	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		ENZYME CONCENTRATION 1 MG/ML, PH 7.0 - 8.5, BUFFER 0.2 M TRIS-HCL, PRECIPITANT PEG 6000 (<10%), 70 MICROMOLAR ZINC SULPHATE, 6 MM BETA-MERCAPTOETHANOL, HANGING DROPS AS FOR PDB ENTRY 1AW5 WITH 10 MM 5-HYDROXYLAEVULINIC ACID IN DROP.