1W2M
Ca-substituted form of E. coli aminopeptidase P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 H |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-17 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 208.359, 312.688, 160.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 182.570 - 2.400 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m35 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.254 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.040 | 0.160 |
Number of reflections | 192310 | |
<I/σ(I)> | 19.6 | 4.5 |
Completeness [%] | 94.5 | 86 |
Redundancy | 2.1 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 25% PEG 4K, 0.1 MM EDTA, 0.1 M TRIS (PH 8.2), 0.2 M NA ACETATE CRYSTALS SOAKED IN 30% ISOPROPANOL PLUS 5 MM CACL2 FOR 10 MIN PRIOR TO FREEZING |