1W1R
Plant Cytokinin Dehydrogenase in Complex with trans-Zeatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Collection date | 2004-10-01 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 171.033, 171.033, 54.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.152 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 63311 | |
| <I/σ(I)> | 11.2 | 2.7 |
| Completeness [%] | 98.9 | 97.9 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | pH 4.60 |
