1W1D
Crystal Structure of the PDK1 Pleckstrin Homology (PH) domain bound to Inositol (1,3,4,5)-tetrakisphosphate
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-05 |
Detector | ADSC CCD |
Wavelength(s) | 0.861,1.040 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.399, 58.922, 36.578 |
Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.500 |
R-factor | 0.147 |
Rwork | 0.145 |
R-free | 0.20000 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.785 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RSPS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.080 | 0.440 |
Number of reflections | 23341 | |
<I/σ(I)> | 17.7 | 3.2 |
Completeness [%] | 98.6 | 99.5 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.08 M MAGNESIUM ACETATE, 0.05M SODIUM CACODYLATE PH 6.5, 30 % PEG 4000 |