1W0H

Crystallographic structure of the nuclease domain of 3'hExo, a DEDDh family member, bound to rAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2004-04-20
Spacegroup name	P 61 2 2
Unit cell lengths	101.600, 101.600, 100.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.590
R-factor	0.17
Rwork	0.168
R-free	0.20200
Structure solution method	OTHER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.590
Rmerge	0.134	0.504
Number of reflections	40933
<I/σ(I)>	20.6	5.4
Completeness [%]	99.5	100
Redundancy	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		pH 5.60