1W0G
Crystal structure of human cytochrome P450 3A4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-26 |
| Detector | RIGAKU RAXIS 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.940, 100.910, 131.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.650 - 2.730 |
| R-factor | 0.238 |
| Rwork | 0.234 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0e |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.753 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0003A) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.890 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.040 | 0.308 |
| Number of reflections | 13241 | |
| <I/σ(I)> | 10.8 | 2.5 |
| Completeness [%] | 94.7 | 97.1 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 0.025M NACL, 7.5% (V/V) ISOPROPANOL, 10 |
