1W08

STRUCTURE OF T70N HUMAN LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-11-15
Detector	MSC
Spacegroup name	P 21 21 21
Unit cell lengths	31.117, 56.178, 62.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 2.500
R-factor	0.18487
Rwork	0.177
R-free	0.26759
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jsf
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.075	0.146
Number of reflections	3903
<I/σ(I)>	14.2	6.5
Completeness [%]	95.1	87.6
Redundancy	6.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	HANGING DROP METHOD AT 293K. DROPLET RESERVOIR SOLUTION MIXED 1:1 WITH 10 MG/ML PROTEIN, 10 MM HEPES BUFFER PH 7.5, 0.4 M LICL. RESERVOIR 2.5 M NACL, 20 MM NAOAC PH 4.5.