1VZE

L. CASEI THYMIDYLATE SYNTHASE MUTANT E60Q TERNARY COMPLEX WITH DUMP AND CB3717

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-10-13
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 61 2 2
Unit cell lengths	78.900, 78.900, 230.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.186
Rwork	0.186
RMSD bond length	0.021
RMSD bond angle	26.340
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.075
Number of reflections	12632
Completeness [%]	54.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	potassium phosphate	20 (mM)
2	1	drop	enzyme	10 (mg/ml)
3	1	drop	ammonium sulfate	15-20 (mM)
4	1	drop	dUMP	5 (mM)
5	1	drop	potassium phosphate	20 (mM)