1VZA

THYMIDYLATE SYNTHASE E60D MUTANT BINARY COMPLEX WITH 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-10-27
Detector	RIGAKU
Spacegroup name	P 61 2 2
Unit cell lengths	79.100, 79.100, 230.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.21
Rwork	0.210
RMSD bond length	0.023
RMSD bond angle	26.940
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.080
Number of reflections	11162 *
Completeness [%]	71.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	potassium phosphate	20 (mM)
2	1	drop	enzyme	10 (mg/ml)
3	1	drop	ammonium sulfate	15-20 (mM)
4	1	drop	dUMP	5 (mM)
5	1	drop	potassium phosphate	20 (mM)