1VZ0
Chromosome segregation protein Spo0J from Thermus thermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9393, 0.9791, 0.9793 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 182.240, 295.150, 73.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.720 - 2.300 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.290 |
| Rmerge | 0.078 | 0.311 |
| Number of reflections | 87653 | |
| <I/σ(I)> | 12.1 | 3 |
| Completeness [%] | 98.3 | 90.6 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.50 |
