1VYM

NATIVE HUMAN PCNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Collection date	2000-02-15
Spacegroup name	C 1 2 1
Unit cell lengths	136.650, 83.265, 71.634
Unit cell angles	90.00, 117.49, 90.00

Refinement procedure

Resolution	14.000 - 2.300
R-factor	0.191
Rwork	0.187
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1axc
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.058	0.450
Number of reflections	31758
<I/σ(I)>	18.4	2.6
Completeness [%]	99.4	98.5
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		20% PEG3350, 0.2M MAGNESIUM ACETATE, pH 7.00