1VYK

CRYSTAL STRUCTURE OF PSBQ PROTEIN OF PHOTOSYSTEM II FROM HIGHER PLANTS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2003-10-15
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	49.938, 49.938, 96.972
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.430 - 1.490
R-factor	0.164
Rwork	0.163
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nze
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.250	1.570
High resolution limit [Å]	1.490	1.490
Rmerge	0.045	0.332
Number of reflections	23594
<I/σ(I)>	9.5	2.2
Completeness [%]	99.7	99.1
Redundancy	9.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	16-18% PEG 6000, 0.1 M MES PH 6.5, 0.3 M ZN ACETATE, VAPOR DIFFUSION, SITTING DROP, TEMP 298K