1VST

Symmetric Sulfolobus solfataricus uracil phosphoribosyltransferase with bound PRPP and GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	120
Detector technology	CCD
Collection date	2005-02-22
Detector	MAR CCD 165 mm
Wavelength(s)	0.954
Spacegroup name	P 64 2 2
Unit cell lengths	122.200, 122.200, 62.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.800
Rwork	0.226
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xtv
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.152	0.418
Number of reflections	7102
<I/σ(I)>	15.9	4.2
Completeness [%]	99.9	99.3
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	10% PEG8000, 0.1M sodium-cacodylate, pH6.5, 200mM magnesium-chloride, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K