1VRA
Crystal structure of Arginine biosynthesis bifunctional protein argJ (10175521) from Bacillus halodurans at 2.00 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-21 |
Detector | ADSC |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.658, 70.658, 222.292 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.810 - 2.000 |
R-factor | 0.14056 |
Rwork | 0.140 |
R-free | 0.15782 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.426 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (5.0)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.810 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.065 | 0.109 |
Number of reflections | 38627 | |
<I/σ(I)> | 21 | 10.2 |
Completeness [%] | 98.8 | 90.7 |
Redundancy | 5.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 4.2 | 277 | 40.0% Ethylene-Glycol, 0.2M (NH4)2SO4, 0.1M Phosphate Citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 4.2 | 277 | 40.0% Ethylene-Glycol, 0.2M (NH4)2SO4, 0.1M Phosphate Citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |