1VR6
Crystal structure of Phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase) (TM0343) from Thermotoga Maritima at 1.92 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.151, 74.226, 249.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.760 - 1.920 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.21519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.760 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.930 |
| Number of reflections | 102464 | |
| <I/σ(I)> | 23.07 | 1.49 |
| Completeness [%] | 98.8 | 90.38 |
| Redundancy | 3.57 | 2.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 5.5 | 293 | 35.0% Ethylene-Glycol, 0.1M Acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K |
