1VR1

Specifity for Plasminogen Activator Inhibitor-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	289
Detector technology	AREA DETECTOR
Spacegroup name	C 1 2 1
Unit cell lengths	70.880, 71.960, 73.040
Unit cell angles	90.00, 100.65, 90.00

Refinement procedure

Resolution	19.900 - 1.900
R-factor	0.204 *
Rwork	0.204
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.700 *
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900
High resolution limit [Å]	1.900	1.930 *
Rmerge	0.094	0.439 *
Number of reflections	24804
Completeness [%]	92.2	81.8 *
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6	6 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.7 (mg/ml)
2	1	reservoir	PEG8000	22-32 (%(w/v))
3	1	reservoir		200 (mM)
4	1	reservoir	Tris-HCl	100 (mM)	pH7.4-8.6