1VQZ
Crystal structure of a putative lipoate-protein ligase a (sp_1160) from streptococcus pneumoniae tigr4 at 1.99 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | ADSC |
| Wavelength(s) | 0.979724,0.956885,0.979691 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.503, 70.280, 113.629 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.100 - 1.990 |
| R-factor | 0.16211 |
| Rwork | 0.159 |
| R-free | 0.21691 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.530 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 25714 | |
| <I/σ(I)> | 14.2 | 3 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 10.4 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.8 | 277 | 0.2M Na2NO3, 20.0% PEG-3350, No Buffer pH 6.8, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
