1VQT
Crystal structure of Orotidine 5'-phosphate decarboxylase (TM0332) from Thermotoga maritima at 2.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-30 |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.688, 85.550, 48.725 |
| Unit cell angles | 90.00, 130.20, 90.00 |
Refinement procedure
| Resolution | 27.000 - 2.000 |
| R-factor | 0.18343 |
| Rwork | 0.181 |
| R-free | 0.22088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eix |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.671 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 17410 | |
| <I/σ(I)> | 28.05 | 1.34 |
| Completeness [%] | 99.5 | 95.32 |
| Redundancy | 4.81 | 3.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.5 | 277 | 12.0% PEG-20000, 0.1M MES pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
