1VQ1
Crystal structure of N5-glutamine methyltransferase, HemK(EC 2.1.1.-) (TM0488) from Thermotoga maritima at 2.80 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-13 |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.973, 58.611, 85.667 |
| Unit cell angles | 90.00, 109.46, 90.00 |
Refinement procedure
| Resolution | 36.090 - 2.800 |
| R-factor | 0.21156 |
| Rwork | 0.208 |
| R-free | 0.27281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nv8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.507 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.090 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 15828 | |
| <I/σ(I)> | 13.3 | 1.7 |
| Completeness [%] | 96.0 | 73.3 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 293 | 5.0% MPD, 10.0% PEG-6000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
