1VPA
Crystal structure of 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TM1393) from Thermotoga maritima at 2.67 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-20 |
| Detector | APS |
| Spacegroup name | P 61 |
| Unit cell lengths | 144.230, 144.230, 53.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.350 - 2.670 |
| R-factor | 0.20234 |
| Rwork | 0.199 |
| R-free | 0.24783 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vgz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.405 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 18105 | |
| <I/σ(I)> | 11.46 | 1.97 |
| Completeness [%] | 98.3 | 98.23 |
| Redundancy | 4.51 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 8 | 277 | 0.2M Ca(OAc)2, 10.0% PEG-8000, 0.1M Imidazole pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
